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KMID : 0379119910190020101
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Korean Journal of Mycology
1991 Volume.19 No. 2 p.101 ~ p.108
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Classification of Isolates of Ganoderma lucidum , Schizophyllum commune and Cordyceps spp. by Electrophoretic Patterns of Isozymes
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Abstract
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To classify fungal species employed for pharmacological effects, mycelial proteins of six isolates of Ganoderma lucidum, five isolates of Schizophyllum commune and five isolates of Cordyceps spp. were separated on polyacrylamide gel to compare them by esterase, acid phosphatase, leucine aminopeptidase and peroxidase patterns. Similarity of isozyme patterns among the isolates of G. lucidum IY003, IY004, IY005 and IY008 was indicated over 70%, but that among the isolates of G. lucidum 1Y009, IY010 and others was indicated from 48% to 9%. Highest similarity of isozymes of S. commune was observed to be between 1Y803 and 1Y805, and similarity between these two isolates was 57%. Similarity among other isolates was shown to be from 40% to 56%. Isozyme patterns of Cordyceps spp. were comparatively different, even though they were originated from the same kind of insect as their isolate. Similarity between Cordyceps spp. IY901 and IY904, which was isolated from moths, was 67% and that of IY905 and IY909, which was originated from the larvae, was 42%. Similarity among other isolates was shown to be from 12% to 67%.
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